People engaging in risky behavior are at risk for contracting HIV infection. Health education programs in schools can reduce the prevalence of such behaviors among students. School policies on HIV can also protect the rights of HIV-infected students and staff and reduce the odds of transmission to others. This report analyzed School Health Profiles from 2006 across 36 states and 13 urban school districts in the U.S. Findings show that the majority of secondary schools had HIV prevention as part of their health education programs, however a minority taught all the topics identified in the questionnaire. Only 50% of schools had policies on HIV-infected pupils or staff. More comprehensive HIV education and policies on HIV infection among students and staff at schools are needed.